Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:

  • Have you selected the most appropriate research strategy?
  • Have you identified the issues critical to your successful application of a technique?
  • Are you familiar with the limitations of a given technique?
  • When should common procedural rules of thumb not be applied?
  • What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:

  • Working safely with biological samples and radioactive materials
  • DNA and RNA purification
  • PCR
  • Protein and nucleid acid hybridization
  • Prokaryotic and eukaryotic expression systems
  • Properly using and maintaining laboratory equipment

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